WSURF Case 749
Protein Interaction Reporters
Summary
Large protein complexes and multi-protein signaling pathways are pervasive in natural systems. Due to the inherently transient nature of protein-protein interactions profiling large-scale interaction networks within a cell present significant challenges. Cross-linking in combination with mass spectroscopy is one commonly used approach, however current methods for utilizing this technique introduce significant complexity in both the cross linking reaction and the resultant mass spectrum. Simple, scalable and broadly applicable analytical methods have yet to be developed for the study of proteome-wide protein-protein interactions. Researchers at WSU have developed a new mass spectrometry-identifiable cross-linking strategy that provides both a simple cross linking reaction and easily interpretable mass spectrum.
Applications & Advantages
- Novel protein crosslinking platform
- Enables MS/MS identification of interaction proteins
- Croslinker selectively cleaved in MS
- Allows for differentiation between dead-end, intra-, and inter- cross-linked peptides
- Selective targeting of protein of interest possible
IP Status
This technology is protected by both a published US Patent application and an additional provisional filing. Exclusive rights are available.
Publications
ASMS 2005 (1)
ASMS 2005 (2)
ASMS 2005 (3)
ASMS 2005 (4)
Chowdhury, Saiful M.; Munske, Gerhard R.; Tang, Xiaoting; Bruce, James E.. Collisionally Activated Dissociation and Electron Capture Dissociation of Several Mass Spectrometry-Identifiable Chemical Cross-Linkers. Analytical Chemistry ACS ASAP
Tang, Xiaoting; Munske, Gerhard R.; Siems, William F.; Bruce, James E.. Mass spectrometry identifiable cross-linking strategy for studying protein-protein interactions. Analytical Chemistry (2005), 77(1), 311-318.
