WSURF Case 883
Isoaspartate Quantitation and Localization Assay
Summary
Long-term storage of a protein often results in the slow modification of it's structure. Among the most prominent structural modification is the appearance of a new amino acid residue: Isoaspartic acid. While there are multiple routes to the formation of isoaspartic acid, it's presence can result in modifications or loss of a proteins activity. Of the many factors that influence the potential effect of isoaspartate formation on a protein's activity and physical properties, the location of the residue within the peptide most certainly factors prominently. While many methods are available in the literature to identify the presence of isoaspartate, none can provide detailed information regarding the location of the non-native residue within the peptide sequence.Researchers at Washington State University have developed a novel method to both quantify and localize the formation of isoaspartic acid.
Applications & Advantages
- Utilizes known enzymes & reagents currently available on the market
- Highly versatile allowing for many different methods of detection
- Provides platform for sample analyte enrichment
- Simple, user friendly
- No Radioactive isotopes necessary
IP Status
The technology is protected by a provisional patent application and is available for exclusive worldwide licensing.
For more information about this technology please contact WSURF:
1610 NE Eastgate Blvd, Suite 650
Pullman, WA 99164
Phone: (509) 335-5526
Fax: (509) 335-7237
wsurf@wsu.edu
